JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963259.1
Database:	nt	Go to original record
Description:	Stapelia gigantea chloroplast, partial genome.
Taxon:	Stapelia gigantea
Original size:	130022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48767	49322	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatt ctgagccaaa tcctattttc aggttcggaa aacgaacaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atagaaaatt tttaatgaca atccaaattc
301   ttccgttttt tctataaaaa acggaagaat tggtgtgatt cgattccaca
351   ttgaagaaag aatcgaatat tcattgatca aaagattcac tccatagtct
401   gtagatcctc ttttcaagaa ccggattaat cggacgagaa taaagaataa
451   agatagagtc ccgttctaca tgtcaatgct ggcaacaatg caattttgag
501   taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details