JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963256.1
Database:	nt	Go to original record
Description:	Mandevilla x amabilis chloroplast, partial genome.
Taxon:	Mandevilla x amabilis
Original size:	128711 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47865	48431	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcctt ccatcgaaaa ttcagaaagg
251   atgaaggata accacgtata gatacgtatt gaatactata tcaaatgatt
301   aatgccgacc cgaatgaatc tctatttttt ctataaaaaa cggaagaatt
351   ggtgtgattc gattctttct tcaatgtgga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg tagatcctct tttcaagaac ggattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatgc tggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga cttacaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details