JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963253.1
Database:	nt	Go to original record
Description:	Amphineurion marginatum chloroplast, partial genome.
Taxon:	Amphineurion marginatum
Original size:	129018 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48032	48597	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata tacatacgta ttgaatacta tatcaaatat caaatgatta
301   atgccgaccc gaatgaatct gtattttttc tataaaaaac ggaagaattg
351   gtgtgattcg attctttctt caatgtggaa tcgaatattc attgatcaaa
401   cgattcactc catagtctgt agatcctctt ttcaagaacg gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgct ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details