JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963250.1
Database:	nt	Go to original record
Description:	Diplorhynchus condylocarpon chloroplast, partial genome.
Taxon:	Diplorhynchus condylocarpon
Original size:	134414 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48592	49150	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aacgcatata catacgtatt gaatactata tcaaatgatt
301   aatgccgacc cgaatgaatc tgtatttgta aaaaatggaa gaattggtgt
351   gattcgattc tttcttcaat gtggaatcga atattcattg atcaaatgat
401   tcactccata gtctgtagat cttttcaaga actgattaat cggatgagaa
451   taaagataga gtcccgttct acatgtcaat gctggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details