JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963248.1
Database:	nt	Go to original record
Description:	Aspidosperma cruentum chloroplast, partial genome.
Taxon:	Aspidosperma cruentum
Original size:	129770 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48661	49221	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc ctgttttcca aaaacaaggg ttcagaaaac
151   gaaaaaagga taggtgcaga gactcaatgg aagctgttct aacaaatgga
201   gttggccgcg ttggtaaaga aatctttcca tcaaaaattc agaaaggatg
251   aaggataaac gtatatacat acgtattgaa tactatatca aatgattaat
301   gacgacccga atgaatctgt attttttcta tgaaaaatgg aagaattggc
351   gtgaatcgat tccacattga agaaagaatc gaatattcat tgatcaaacg
401   attcactcca tagtctgtag atcttttcaa gaactgatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca atgccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details