JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963245.1
Database:	nt	Go to original record
Description:	Kopsia rosea chloroplast, partial genome.
Taxon:	Kopsia rosea
Original size:	126663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47643	48207	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaagcaaa ggttcagaaa
151   acgaaaaagg gataggtgca gagacttaac ggaagttgtt ctaacaaatg
201   gagttggccg cgttggtaga aaaatctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaatgatta
301   atgcggacct gaatgaatct gtattttttc tagaagaatt ggtgtgattc
351   gatttcacat tgaagaaatt gaagaaagag tcgaatattc tcattgatca
401   aatgattcac tccatagtct atagatcttt tcaagaactg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gttaatgctg gcaacaatga
501   aatttagagt aagaggaaaa tccgtcgact taaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details