JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963243.1
Database:	nt	Go to original record
Description:	Hunteria zeylanica chloroplast, partial genome.
Taxon:	Hunteria zeylanica
Original size:	110978 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48132	48693	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcgga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcttggtaga gaaatctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaatgatta
301   atgccgaccc gaatgaatct gtatttttct ataaaaaatg gaagaattgg
351   tgtgattcga ttccacattg aagaaagaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgta gatcttttca agaactgatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aatgctggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttaa aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details