JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963242.1
Database:	nt	Go to original record
Description:	Secondatia densiflora chloroplast, partial genome.
Taxon:	Secondatia densiflora
Original size:	129939 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48438	49003	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   agggcaatcc tgagccaaat cctgttttcc acaaacaaag gttcagaaaa
151   cgaaaacaag gataggtgca gagactcgac ggaagctgtt ctaacaaatg
201   gagttggcca cgttggtaga gaaatctttc catcgaaaat taagaaagga
251   tgaaggataa acacatatac atacgtattg aatactatat caaatgatta
301   atgccgcccc gaatgaatct gtattttttc tataaaaaac ggaagaattg
351   gtgtgattcg attctttctt caatgtggaa tcgaatattc attgatcaaa
401   tgattcaccc catagtctgt agatcctctt ttcaagaacg gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatgct ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details