JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963240.1
Database:	nt	Go to original record
Description:	Thevetia peruviana chloroplast, partial genome.
Taxon:	Thevetia peruviana
Original size:	128309 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46913	47511	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaactcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgtgttc cataaacata aacaagggtt
151   cagaaaacga aaacaaggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttagccgcgt ttctttccat cgaaaattca gaaaggatga
251   aggataaaca cgtatataca tacgtattga atactatatc aaatgattaa
301   tgccgacccg aatgaatctg tattttttct ataaaaatgg aagaattggt
351   gttattcgat tccacattga agaaaggaat cgaatattca ttgatcaaat
401   acattgaaga aaggaatcga atattcattg atcaaatgat tcactcccta
451   gtctgtagat cttttcaaga actgattaat cggacgagaa taaagataga
501   gtcccgttct acatgtcaat gctggcaaca atgaaattta tagtaagagg
551   aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details