JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963239.1
Database:	nt	Go to original record
Description:	Streptoechites chinensis chloroplast, partial genome.
Taxon:	Streptoechites chinensis
Original size:	129366 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48765	49326	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgataacttt caaattcaga aaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttcgttg gtagagaaat ctttccatcg aaaattcaga
251   aaggatgaag gatatacata cgcattgaat actatatcaa atgattaatg
301   ccgacccgaa tgaatctgta ttttttctat aaaaacggaa gaattggtgt
351   gattcggttc tttcttcaat gtggaatcga atattcattg atcaaacgat
401   tcactccata gtctgtagat cctcttttca agaacggatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aatgctggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttaa aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details