JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963238.1
Database:	nt	Go to original record
Description:	Alafia barteri chloroplast, partial genome.
Taxon:	Alafia barteri
Original size:	129264 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48287	48853	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcggaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttgacc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aacacatata catacgtatt gaatactata tcaaatgatt
301   aatgccgacc cgaatgaatc tgtatttttt ctataaaaaa cggaagaatt
351   ggtgtgattc gattctttcc tcaatgtgga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg tagatcctct tttcaagaac ggattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatgc tggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga cttaaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details