JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963234.1
Database:	nt	Go to original record
Description:	Ambelania acida chloroplast, partial genome.
Taxon:	Ambelania acida
Original size:	125673 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48956	49540	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagacttaac ggaagctgtt ctaacaaatg
201   gagttggccg cgttggtaga gaaacctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat gaaatgatta
301   atatgaaatg attaatgatt aatgccgacc cgaatgaatc cgtatttttt
351   ctataaaaaa tagaagaatt ggtgtgattc gattccccat tgaagaaaga
401   atcgaatatt cattgatcaa ataattcact ccatagtctg tagatctttt
451   caagaactga ttaatcggac gagaataaag atagagtccc gttcttacat
501   gtcaatgctg gcaacaatga aatttatagt aagaggaaaa tccgtcgact
551   taaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details