JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963233.1
Database:	nt	Go to original record
Description:	Leuconotis anceps chloroplast, partial genome.
Taxon:	Leuconotis anceps
Original size:	124585 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48380	48950	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaaagg gataggtgca gagactcgac ggaagctgtt ctaacaaacg
201   gagttggccg cgttggtaga gaaacctttc catcgaaaat taagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaatgatta
301   atgccgaccc gaatgaatct gtattttttc ttttttctat aaaaaaatgg
351   aagaattggt gtgattcgat tccacattga agaaagaatc gaatattcat
401   tgatcaaatg attcactcca tagtctgtag atcttttcaa gaactgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca atgctggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgatttaaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details