JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963232.1
Database:	nt	Go to original record
Description:	Allamanda schottii chloroplast, partial genome.
Taxon:	Allamanda schottii
Original size:	128603 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47986	48572	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttt cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccattgaaaa ttcagaaagg
251   atgaaggata aacgtatata catacgtatt gaatattgaa tattgaatac
301   tatatcaaat gattaatgcc gacccgaatg aatctgtatt tttctataaa
351   taatggaaga attggtgtga ttcgattctt tcttcaatgt ggaatccaat
401   attcaatgat caaatgattc actccatagt ctgtagatct tttcaaatct
451   tttcaagaac tgattaatcg gacgagaata aagatagagt cccgttctac
501   atgtcaatgc tggcaacaat gaaatttata gtaagaggaa aatccgtcga
551   cttaaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details