JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963230.1
Database:	nt	Go to original record
Description:	Mesechites trifidus chloroplast, partial genome.
Taxon:	Mesechites trifidus
Original size:	128408 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48285	48825	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aaagggcaat cctgagccaa atcctatttt ccacaaacaa aggttcagaa
151   aacgaaaaca aggataggtg cagagactcg acggaagctg ttctaacaaa
201   tggagttggc cgcgttggta gagaaatctt tccatcgaaa attcagaaag
251   gatgaaggat aaacacatat acatacgtat tgaatactat atcaaatgat
301   taatgccgac ccgaatgaat ctctattttt tctataaaaa aaggaagaat
351   tggtgtgatt cgattctttc ttcaatgatt cactccatag tctgtagatc
401   ctcttttcaa gaacggatta atcggacgag aataaagata gagtcccgtc
451   ctacatgtca atgctggcaa caatgaaatt tatagtaaga ggaaaatccg
501   tcgacttaaa aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details