JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963228.1
Database:	nt	Go to original record
Description:	Vinca major chloroplast, partial genome.
Taxon:	Vinca major
Original size:	127690 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47387	47961	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataatttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttt cacaaacaaa gaaacaaagg
151   ttcagaaaac gaaaaaaagg ataggtgcag agactcgacg gaagctgttc
201   taacaaatgg agttggccgc gttggtagag aaatctttcc attgaaaatt
251   aagtgaagta taaacgtata tgcatacgta ttgaatacta tatctactat
301   atcaaatgat taatgccgac ccgaatgaat ctgtagtttt tctctaaaaa
351   atggaagaat tgatgtgatt cgattccaca ttcaagaaag aatcaaatat
401   tcattgatca aatgattcac tccatagtct gtagatcttt tcaagaactg
451   attaatcgga caagaataaa gatagagtcc cgttctacat gtcaatgctg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact taaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details