JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963227.1
Database:	nt	Go to original record
Description:	Pycnobotrya nitida chloroplast, partial genome.
Taxon:	Pycnobotrya nitida
Original size:	128810 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48368	48930	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaataaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggccg cgttggtaga gaaacctttc catcgaaaat tcagaaaggg
251   tgaaggataa acgtatatac atatgtattg aatactatat caaatgatta
301   atgccgaccc gaatgaatct gtattttttc tataaaaaat ggaagaattt
351   gtgtgattcg attccacatt gaagaaagga tcgaatattc attgatcaaa
401   tgattcactc catagtctgt agatcttttc aagaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatgctggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgactta aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details