JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963225.1
Database:	nt	Go to original record
Description:	Malouetia tamaquarina chloroplast, partial genome.
Taxon:	Malouetia tamaquarina
Original size:	127403 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49048	49613	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aacacatata catacgtatt gaatactata tcaaatgatt
301   aatgccgacc cgaacgaatc tgtatttttt ctataaaaaa cggaagaatt
351   ggtgtgattc gattctttct tcaatgtgga atcgaatatt cattgatcaa
401   atgattcact ccatagtccg agatcctctt ttcaagaacg gattaatcgg
451   acgagaataa agatagagtc ccgttctacg tgtcaatgct ggcaacaatg
501   aaatttagag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details