JRC GMO-Amplicons | EURL GMFF

Record ID:	MG831879.1
Database:	nt	Go to original record
Description:	Sampaiella trichoclada chloroplast, partial genome.
Taxon:	Sampaiella trichoclada
Original size:	155506 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46567	47131	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aaggaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcgttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   atgaaggata aacgtatcta ttgaatacta tatcaataga ttaatgatgg
301   cccgaatctg tatctgtatt tttttatatt atatgaaaaa tggaagaatt
351   agtgtgaatt gattccacat tgatgaaaga atcgaatatt cattcatcaa
401   atcattcact ccatagtccg atagttcttt taaagaactg attaagcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaagaatga
501   aatttatagt aagaggaaaa tccgtcgact tttaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details