JRC GMO-Amplicons | EURL GMFF

Record ID:	MG831842.1
Database:	nt	Go to original record
Description:	Adenocalymma adenophorum chloroplast, partial genome.
Taxon:	Adenocalymma adenophorum
Original size:	142857 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	40629	41192	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aaggaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gccttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   atgaaggata aacgtatcta ttgaatacta tatcaataga ttaatgatag
301   cccgaatctg tatctgtatt ttttttatag tatatgaaaa atggaagaat
351   tagtgtgaat tgattacaca ttgaagaaag aatcgaatat tcattcatca
401   aatttcactc catagtccga tagttctttt aaagaactga ttaagcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt ttaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details