JRC GMO-Amplicons | EURL GMFF

Record ID:	MG831839.1
Database:	nt	Go to original record
Description:	Adenocalymma ackermannii chloroplast, partial genome.
Taxon:	Adenocalymma ackermannii
Original size:	150778 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44731	45296	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt cttaaaacaa aggttcagaa
151   aaggaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gccttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   atgaaggata aacgtatcta ttgaatacta tatcaataga ttagtgatag
301   cccgaatctg tatctgtatt ttttttatag tatatgaaaa atgggagaat
351   tagtgtgaat tgattacaca ttgaagaaag aatcgaatat tcattcatca
401   aatcattcac tccatagtcc gatagttctt ttaaagaact gattaagcgg
451   acgagaataa agatagagtc ccattctaca tgtcaatacc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttttaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details