JRC GMO-Amplicons | EURL GMFF

Record ID:	MG831865.1
Database:	nt	Go to original record
Description:	Adenocalymma scabriusculum chloroplast, partial genome.
Taxon:	Adenocalymma scabriusculum
Original size:	158482 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46923	47490	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aaggaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgccttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaatag attagtgata
301   gcccgaatct gtatctgtat ttttttttat agtatatgaa aaatggaaga
351   attagtgtga attgattaca cattgaagaa agaatcgaat attcattcat
401   caaatcattc actccatagt ccgatagttc ttttaaagaa ctgattaagc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg acttttaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details