JRC GMO-Amplicons | EURL GMFF

Record ID:	MF460830.1
Database:	nt	Go to original record
Description:	Anemopaegma arvense voucher F. Firetti 237 chloroplast, complete genome.
Taxon:	Anemopaegma arvense
Original size:	168839 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47477	48046	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aaggaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttagc tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct atctattgaa tactatatca aatgattaat
301   gatggcccga atctgtatct gtattttttt atattatatg aaaaatggaa
351   gaattagtgt gaattgattc cacattgaag aaagaatcga atattcattc
401   atcaaatcat tcactccata gtccgatagt tcttttaaag aactaattaa
451   gcggacgaga ataaagatag agtcccattc tacatgtcaa taccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgacttttaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details