Match details

Record ID: KY703615.1
Database: nt Go to original record
Description: Cauliflower mosaic virus strain DSMZ PV-0362, complete genome.
Taxon: Cauliflower mosaic virus
Original size: 8010 bp

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

View other 15625 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 0 7218 7299 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc taaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc ccaacacgtc tt 

View other 3 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
probe 100.0 21 bp 0 0 22 42 1.28e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.