JRC GMO-Amplicons | EURL GMFF

Record ID:	LT558590.1
Database:	nt	Go to original record
Description:	Brachypodium distachyon chloroplast genome, ecotype Adi2
Taxon:	Brachypodium distachyon
Original size:	135186 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46441	47056	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tagtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattta tttaggttgt tttggtagtg gaaatccttt
251   taaattagag aaagaaggga tttttacatc taataaacac gtatagatac
301   taacatagta aacgattaat cacagaatcc aagtataaca taggttcttt
351   attctttttt agaatgaaat taggaaggat tatgaaataa aaaattaata
401   aattttttag aattattgtg aatccattct aattgaatct tgagtaatca
451   aatccttcaa ttcaaagtac ttgagatctt ttaaaaagtg gattaatcgg
501   acgaggacaa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttcgag taaaaggaaa atccgtcgac tttataagtt gtgagggttc
601   aagtccctct atcccc

View other 87 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details