JRC GMO-Amplicons | EURL GMFF

Record ID:	KY432797.1
Database:	nt	Go to original record
Description:	Stipagrostis hirtigluma voucher K:MSB 74902 chloroplast, complete genome.
Taxon:	Stipagrostis hirtigluma
Original size:	138052 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46907	47517	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tcttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatgg aggtaattac gttgtgttgg tagtggacct ccctctaaat
251   tagagaaaga ggggctttat acatctaata cacacgtata tatactgaca
301   tagcaaacga ttaatcacag aacccatatc ataatatagg ttctttattt
351   atttagaatt tagaatgaaa ttaggaatga ttatgaaatt aaaaattctg
401   aatttttttt aattattgtg aatccaatcg aatattgagt aatcaaatcc
451   ttcaattcat tgttttcgag atcttttaaa aagtggatta atcggacgag
501   gataaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details