JRC GMO-Amplicons | EURL GMFF

Record ID:	KY189293.1
Database:	nt	Go to original record
Description:	Stephania longipes isolate S2014047 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Stephania longipes
Original size:	912 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	579	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagccc ttggtatgga
51    aacctgctaa gtgaaaactt tcaaattcag agaacccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctgttttca gaaaacaaac aaaaaggggt
151   tcagaaaagg agactcaaaa gtggataggt gcagagactc aatggaagct
201   gttctaagaa atggagttga ttgcgttgcg ttagtggagg aatccttcta
251   gtgaaactcc agaaaggatg aacttatcta tatacatacg tctacgtact
301   gaaatatcaa agattcatga tgatccgaat ctgtatttat gaaaaatgga
351   agaatttttg tttttgtaaa tcaattccaa gttgaaggaa gaatcgaaca
401   ttcactgatc aaatcagtca ctccatagtc tgataggtct tttgaagaac
451   tgactaatcg gacgagaata aagatagagt cccattctac gtgtcaatac
501   cgacaacaat gaaatttata gtaagaggaa aatccgtcga ctttagaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details