JRC GMO-Amplicons | EURL GMFF

Record ID:	LC199758.1
Database:	nt	Go to original record
Description:	Primula takedana chloroplast DNA, tRNA-Leu, trnL-trnF intergenic spacer, tRNA-Phe, partial sequence, isolate: TA3.
Taxon:	Primula takedana
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcctctttt tcgaaaacaa aggtgaaagg
151   aaaatcaaaa agggggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt ggtcgcgttg gtagaggaat cctttcatga aaattgcaga
251   taaggatgaa acagaaatgt atatacatac gcatatgtac tgaaatacta
301   tatcatcaaa ataaaatgtt tattttttct ataaaaaaaa gagaaaaata
351   gaagaattgt tgcgaatcga ttctacattc cacattgact aaagaatttc
401   atattcattg atcaaagcat ttactccata ccataatagt ctgatagatc
451   ttgtgaataa ctgattaatc aggcgagaat aaagatagag tcccattcta
501   catgtcaata tcgacaacaa taatgaaatt tatagtaata ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details