JRC GMO-Amplicons | EURL GMFF

Record ID:	MF472011.1
Database:	nt	Go to original record
Description:	Primulina eburnea chloroplast, complete genome.
Taxon:	Primulina eburnea
Original size:	152373 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46956	47506	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagtgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ccacgttggt agaagaattg aaacttcaga aaggatgaag
251   gataaacgta tctattgaat actattgatg attaataatg gcccgaatct
301   gtatctgggt attttctata tgaaaaatag aagaattggt gtgaatttat
351   ttcacattga taaaagaatc gaatatttat tagtcaaatc attcactcca
401   cagtccgata gatctcttaa agaacttatt attcggacga gaataaagat
451   agagtcccgt tctaatgtca ataccggcaa caatgaaact tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details