JRC GMO-Amplicons | EURL GMFF

Record ID:	AP017909.1
Database:	nt	Go to original record
Description:	Rumex nepalensis chloroplast DNA, nearly complete sequence.
Taxon:	Rumex nepalensis
Original size:	158777 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	51135	51730	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtacggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tcctcctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta gtagttatta taagttataa gaattcttcc atcgaaactc
251   caaaaaagat gaagaataaa cctatccgca gacgtactta aatataaata
301   ctatataawt attaaattaa aaaatattga tgacgacccg cattttttta
351   tgactatgaa aagatggaag aattgttgtg aagcgattcc aagttgaaga
401   aagaatcgac tattcgttta ataaattaaa gcatttattc cctgaagtct
451   gatagatctt ttgaagaacc gatccatcgg atgagaatga agatagagtc
501   ccattctacg tgtcaatcct gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details