JRC GMO-Amplicons | EURL GMFF

Record ID:	AP017907.1
Database:	nt	Go to original record
Description:	Tricyrtis macropoda chloroplast DNA, nearly complete sequence.
Taxon:	Tricyrtis macropoda
Original size:	153981 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45703	46295	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaagta aattgagcct tagtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga taaaacaagg atttaatttc
151   tcaaactaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg aagtttatta cgttacgttg gtagctggaa tccttctatc
251   aaaattacgg agaggatgcc tgtatacgta tatatacata ctgacatagt
301   aaacaattga ttattgatta atcgtggccc aaatccatta tatatatata
351   tgaaaaaatt gagagttatt gtgaatctct tccaatcgaa attgaaggaa
401   gaatcgaata ttcatattca gtgatcaaat tattcattcc agagtctgat
451   agatcttttg aaaaacggat tattcggacg agaataaaga gagagtccca
501   ttttacgtgt caataccgac aacaatgaaa tttatagtaa aaggaaaatc
551   cgtcgacttt ataagtcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details