JRC GMO-Amplicons | EURL GMFF

Record ID:	KT897823.1
Database:	nt	Go to original record
Description:	Colchicum micranthum voucher E. Kaya_C3404 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colchicum micranthum
Original size:	1005 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatgttaa gaaaacaaag gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gaattgacgt tgactacgtt
201   gcgttggtag ctggaatccc tctatcgaaa ttactaatca caactcgaat
251   ctggaatcca tattattaac tataactata tattatatat attatagtta
301   ttattatata tattttttta tattttaaaa aagatatatt tttatatcaa
351   tttatagatt aattaagagt tattataaat atattccagt cgaagttgaa
401   ggaagaatcg aatattcagt attcagtgat caaatcattc attccggagt
451   cggatagatc ttttgaaaaa atgattaatc ggacgagaat aaagagagag
501   tcctgttcta cgtgtcaata ccgacagcaa tgaaatttat agtattagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details