JRC GMO-Amplicons | EURL GMFF

Record ID:	KT897783.1
Database:	nt	Go to original record
Description:	Colchicum bivonae voucher E. Kaya_C1703 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colchicum bivonae
Original size:	992 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	580	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatgttaa gaaaacaaag gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gaattgatgt tgactacgtt
201   gcgttggtag ctggaatccc tctatcgaaa ttacagaaag gatgacccta
251   tatgcgtata tatacatact gacatatcaa acgattaatc acaactcttt
301   tatattttta aaaagatata tttttatata aatttataga ttaattaaga
351   gttattataa atatattcca gtcgaagttg aaggaagaat cgactattca
401   gtattcagtg atcaaatcat tcattccgga gtcggataga tcttttgaaa
451   aaatgattaa tcggacgaga ataaagagag agtcctgttc tacgtgtcaa
501   taccgacagc aatgaaattt atagtattag gaaaatccgt cgactttaga
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details