JRC GMO-Amplicons | EURL GMFF

Record ID:	KT897743.1
Database:	nt	Go to original record
Description:	Colchicum sanguicolle voucher E. Kaya_C0717 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colchicum sanguicolle
Original size:	1022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	618	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatgttaa gaaaacaaag gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gaattgacgt tgactacgtt
201   gcgttggtag ctggaatccc tctatcgaaa ttacagaaag gatgacccta
251   tatgcgtata tatacatact gacatatcaa acgattaatc acaactcgaa
301   tctggaatcc atattattaa ctataactat atattatagt tattattata
351   gatatatttt tatataaatt tatagattaa ttaagagtta ttataaatat
401   attcctagtc gaagttgaag gaagaatcga atattcagta ttcagtgatc
451   aaatcattca ttccggagtc ggatagatct tttgaaaaaa atgattaatc
501   ggacgagaat aaagagagag tcctgttcta cgtgtcaata ccgacagcaa
551   tgaaatttat agtattagga aaatccgtcg actttagaaa tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details