JRC GMO-Amplicons | EURL GMFF

Record ID:	MF765599.1
Database:	nt	Go to original record
Description:	Utricularia paulineae tRNA-Leu (trnL) gene and trnL-trnF spacer region intergenic spacer region, partial sequence; chloroplast.
Taxon:	Utricularia paulineae
Original size:	929 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	560	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaaaacttt caaattcagg gaaaccccgg acttaataaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaagaa aggttcagaa
151   aacgaaaaaa gggataggat aggtccagag actcaatgga agctgttcta
201   acaaatgggc actggtatag gaatctttcc accgaaacgg cagaaaggat
251   caaggataaa cctatctatt gaatactata tcaaatgatt aaagatggcc
301   cgaatctcta gatgtctttt tttgatatca aaaaaagaag aattggtgtt
351   aattcattcg acattgaata aaaaatagaa tttttattca tcaaaaaatt
401   aactccctat tccgataaac cttttaaaga actgatcaat cggacaagaa
451   taaagataga gtccccttct acatgtcaat accggcaaca atgaaattta
501   tagtaagagg aaaatccgtt gacttttaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details