JRC GMO-Amplicons | EURL GMFF

Record ID:	MF765585.1
Database:	nt	Go to original record
Description:	Utricularia laxa tRNA-Leu (trnL) gene and trnL-trnF spacer region intergenic spacer region, partial sequence; chloroplast.
Taxon:	Utricularia laxa
Original size:	905 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	538	+	537 bp

Amplicon:

537 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaacccggg aattcacaaa
101   aatgggcaat cctgagccaa atcctgtttt ttcaaaagaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtccagagac tcaatggaag ctgttctaac
201   aaatgggggc accggtagag gaatctttgc accaaaacaa aacgtcagaa
251   taaaaaatca aatgattaat aatggcccga atctcgagat gtttttttta
301   tatgaaaaat agaagcagga ttggtgtgaa ttcattcgac attcacgaaa
351   aaatagaatt gttattcatc aaatcattca ctccatagtc cgataaacct
401   tttaaagaac tgatcaatcg gacaagaata aagatagagt ccccttctac
451   atgtcaatac cggcaacaat gaaatttata gtaagaggaa aatccgttga
501   ctttttaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	537	518	2.42e-08

Match details