JRC GMO-Amplicons | EURL GMFF

Record ID:	KX872894.1
Database:	nt	Go to original record
Description:	Vahlodea atropurpurea voucher CMN:590732 (P.M. Peterson, J.M. Saarela & S.F. Smith 18728) tRNA-Leu (trnL-CAA) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF-GAA) gene, partial sequence; chloroplast.
Taxon:	Vahlodea atropurpurea
Original size:	1010 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	619	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacccatat tataatatag gttctttatt
351   cttttttaga atgaaatttg aaatagaaat gattatgaaa taaaaaattc
401   agaatttttt tagaattatt ttgaatccat tccaagcgaa tatttagtaa
451   tcaaatcctt caattcaaag ttttgaggtc tttcaaaaag tggattaatc
501   ggacgaggac aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttat agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details