JRC GMO-Amplicons | EURL GMFF

Record ID:	MF563382.1
Database:	nt	Go to original record
Description:	Coelachne africana voucher K:RCH09 chloroplast, complete genome.
Taxon:	Coelachne africana
Original size:	135161 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46793	47410	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccacttttga aaaacaagcg gttctcaaac
151   tagaaaaagg ataggtgcag agactcaatg gaagctgttc taacgaatcg
201   aggtaattac gttgtgttgg tagtgaaact ccctctaaat tagagaaaga
251   agggctgtat acctctaata cacacgtata gatactgaca tagcaaacca
301   aacgattaat cacagaatcc tatatcataa tataggttct ttatttattt
351   ttattttctg aaattcagaa taattttgaa ataaaattaa ataatttttt
401   tagaattttt agaattattc ggaatcaatt ctagtcgaac attgagtaat
451   caaacccttc aattcattgt ttttgagatc ttttaaaaag tggattaatc
501   ggacgaggat aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag ttgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details