JRC GMO-Amplicons | EURL GMFF

Record ID:	LC206373.1
Database:	nt	Go to original record
Description:	Oxalis acetosella chloroplast gene for tRNA-Leu, trnL-trnF intergenic spacer, partial sequence, isolate: 347.
Taxon:	Oxalis acetosella
Original size:	990 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	570	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctcttttccg aaaacaaaca aagttttcga
151   aagcgagaat aaaaaaggat aggtgcagag actcaacgga agctattcta
201   aaatttaaaa atagatttta ctgcgttgcg ttagtaaata aacccttccc
251   tcaaaattct agaaaggata aacctatata catacgtata cgtactgaaa
301   tactatttca aatgattaat gatgacccaa atctgtatta tttatcaaaa
351   aaaatgaaag atttgttgtg aatcgattcc aagttgaaga aagaatcgaa
401   tgataattga tcaaatcatt cactccagtg tcggatagat cttttgaaaa
451   acggatagaa taaagataga gtcccattct acatgtcaat attgacaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttagaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details