JRC GMO-Amplicons | EURL GMFF

Record ID:	KX984959.1
Database:	nt	Go to original record
Description:	Taraxacum coreanum var. flavescens isolate Kor-Cheonan tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Taraxacum coreanum var. flavescens
Original size:	935 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	521	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag gtgataaact tttcaaattc agagaaaccc ctggaattaa
101   taaaaatggg caatcctgag ccaaatcacg ttttccgaaa aacaaacaaa
151   gattcagaaa gcgaaaataa aaaaggatag gtgcagagac tcgatggaag
201   ctgttctaac gaatggagtt tattgtctta cgttaataga ggaatccttc
251   tatcgaaact tcagaaaaga tgaaggataa acctgtagac ataatacaga
301   agaattgttg tcaatcgatt ccatattgaa gaaagaatcg aatattcatt
351   gataaaacca ttcactccat aatctgatag atcttttgaa gaactgatta
401   atcggacgag aataaagata gagtcccgtt ctacatgtca ataccggcaa
451   caatgaaatt tatagtaaga ggaaaatccg tcgatttcaa aaatcgtgag
501   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details