JRC GMO-Amplicons | EURL GMFF

Record ID:	MF536701.1
Database:	nt	Go to original record
Description:	Cucumis melo var. momordica isolate MR-1 chloroplast, complete genome.
Taxon:	Cucumis melo var. momordica
Original size:	155815 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49404	50008	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttttttccg aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgacgac gttgcgttag
201   taaaggaatg aatccttcca tcgaaactcc agaaacgatg aaagataaac
251   gtattacgta ctgaaatact atattgatta atgacaactc gaatctctat
301   tttttatatt tctataattt tttatattta tatttatata tatatataaa
351   attatatata aaaatgaacg aattgttatg aatcgattcc aagatctaca
401   agttgaaaaa ataatcgaat attcattgat caaatcattt actccatcat
451   agtctgatag atcttttgaa gaattgatta atcagatcga atagaataaa
501   gatagagtcc cattctacat gtcaataacg acaaaaatga aatttatagt
551   aagaggaaaa tccgtcgact tttaaaatcg tgagggttca agtccctcta
601   tcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details