JRC GMO-Amplicons | EURL GMFF

Record ID:	MF536696.1
Database:	nt	Go to original record
Description:	Cucumis melo subsp. agrestis isolate C-836 chloroplast, complete genome.
Taxon:	Cucumis melo subsp. agrestis
Original size:	156035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49420	50012	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttttttccg aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgacgac gttgcgttag
201   taaaggaatg aatccttcca tcgaaactcc agaaacgatg aaagataaac
251   gtattacgta ctgaaatact atattgatta atgacaactc gaatctctat
301   tttttatata tatatttata tatatatata taaaattata tataaaaatg
351   aacgaattgt tatgaatcga ttccaagatc tacaagttga aaaaataatc
401   gaatattcat tgatcaaatc atttactcca tcatacatag tctgatagat
451   cttttgaaga actgattaat cagatcgaat agaataaaga tagagtccca
501   ttctacatgt caataacgac aaaaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details