JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972896.1
Database:	nt	Go to original record
Description:	Vatica vinosa isolate 01-0248 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vatica vinosa
Original size:	1819 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	830	1410	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   gggcaatcct gagccaaatc ctgtttaagt tcaaaaagaa ataggatagg
151   tgcagagact caatggaagt tgttcgaaca aatggggttg actgcttttg
201   cgttggtaaa agaatacttc tatcgaaact tcagcaagga taaacatata
251   gaatacataa tgaaaaaact atttcaaaaa agacgactgg gacacctatt
301   cttttgattt ctattttttt ataggttata gcaaaaagaa ttatatgaaa
351   aatcaaaaga atccttgtga atcgatttca aattgaagaa agaatcaaat
401   agaatattca ctaatcaaac aattcactcc atagtctgat aaatcaatgg
451   attaattgga cgagaataaa gatagagtcc cattctacat gtcaatatca
501   ataccgacaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgtgag ggttcaagtc cctctatccc c

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details