JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972881.1
Database:	nt	Go to original record
Description:	Vatica harmandiana isolate 207565 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vatica harmandiana
Original size:	1813 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	811	1391	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaagtt caaaaagaaa taggataggt
151   gcagagactc aatggaagtt gttcgaacaa atggggttga ctgcttttgc
201   gttggtaaaa gaatacttct atcgaaactt cagcaaggat aaacatatag
251   aatacataat gaaaaaacta tttcaaaaaa gacgattggg acacctattc
301   ttttgatttc tatttttttt ataggttata gcaaaaagaa ttataggaaa
351   aatcaaaaga atcgttgtga atcgatttca atttgaagaa agaatcaaat
401   agaatattca ctaatcaaac aattcactcc atagtctgat aaatcaatgg
451   attaattgga cgagaataaa gatagagtcc cattctacat gtcaatatca
501   ataccgacaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details