JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972880.1
Database:	nt	Go to original record
Description:	Vatica diospyroides isolate SDDip2014_05 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vatica diospyroides
Original size:	1813 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	811	1392	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   gggcaatcct gagccaaatc ctgtttaagt tcaaaaagaa ataggatagg
151   tgcagagact caatggaagt tgttcgaaca aatggggttg actgcttttg
201   cgttggtaaa agaatacttc tatcgaaact tcagcaagga taaacatata
251   gaatacataa tgaaaaaact atttcaaaaa agacgattgg gacacctatt
301   cttttgattt ctattttttt tataggttat agcaaaaaga attataggaa
351   aaatcaaaag aatcgttgtg aatcgatttc aatttgaaga aagaatcaaa
401   tagaatattc actaatcaaa caattcactc catagtctga taaatcaatg
451   gattaattgg acgagaataa agatagagtc ccattctaca tgtcaatatc
501   aataccgaca acaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details