JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972873.1
Database:	nt	Go to original record
Description:	Vateria copallifera isolate RSD-216 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vateria copallifera
Original size:	2038 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1030	1616	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   gggcaatcct gagccaaatc ctgtttaaaa aagcgttcaa aaagaaatag
151   gataggtgca gagactcaat ggaagttgtt cgaacaaatg gggttgactg
201   cttttgcgtt ggtaaaagaa tacttctatc gaaacttcag caaggataaa
251   catatagaat acataatgaa aaaactattt caaaaaagac gactgggaca
301   cctattcttt tgatttctat ttttttatag gttatagcaa aaagaattat
351   atgaaaaatc aaaagaatcg ttgtgaatcg atttcaaatt gaagaaagaa
401   tcaaatagaa tattcactaa tcaaacaatt cactccatag tctgataaat
451   caatggatta attggacgag aataaagata gagtcccatt ctacctgtca
501   atatcaatac cgacaacaaa gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details