JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972770.1
Database:	nt	Go to original record
Description:	Sarcolaena sp. JH-2017 isolate 903 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Sarcolaena sp. JH-2017
Original size:	1997 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	976	1573	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   gggcaatcct gagccaaatc ctgttttacg cgaaaagggt tcaaaaagca
151   ataggatagg tgcagagact caatggaagt tgttcgaaca aatggggttg
201   actgcttttg cgttggtaaa agaatacttc tatcgaaact ccagtaagga
251   taaacatata gaatacgtaa tgaaaaacta tctcaaaaaa gacgactgag
301   acacctattc ttttgattta tattttttta taggttatag caaaaagaat
351   tatatgaaaa atcaaaagaa tcgttgtgaa tcgattccaa attgaagaaa
401   gaatctaata gaatattcat taatcaaatc attcactcca tagtctgata
451   aatcctttga aaaatagatt aatcggacga gaataaagat agagtcccgt
501   tctacatgtc aatatcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details