JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972759.1
Database:	nt	Go to original record
Description:	Leptolaena multiflora isolate 241 trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; plastid.
Taxon:	Leptolaena multiflora
Original size:	1869 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	901	1501	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggggcaatcc tgagccaaat cctgttttac gcgaaaaggg ttcaaaaagc
151   aagaggatag gtgcagagac tcaatggaag ttgttcgaac aaatggggtt
201   gactgctttt gcgttggtaa aagaatactt ctatcgaaac tccagtaagg
251   ataaacatat agaatacgta atgaaaaact atctcaaaaa agacgactga
301   gacacctatt cttttgattt atattttttt ttttaggtta tagcaaaaag
351   aattatatga aaaatcaaaa gaatcgttgt gaatcgattc caaattgaag
401   aaagaatcga atagaatatt cattaatcaa atcattcact ccatagtctg
451   ataaatcctt tgaaaaatag attaatcgga cgagaataaa gatagagtcc
501   cgttctacat gtcaaaatca ataccgacaa caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details