JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972739.1
Database:	nt	Go to original record
Description:	Helianthemum obscurum isolate JH275 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Helianthemum obscurum
Original size:	1956 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	935	1521	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agcaattcaa aaaggaatca
151   aaaataggat aggtgcagag actcgatgga agttgttcga acaaatggga
201   ttgaatggct ttgcgttagt aaaagaatac ttttattgaa actcaagcaa
251   ggctaaacat atagaataaa ctatttcaaa aaaaaaagag gaatggggca
301   cctattcttt tgatttgctt ataggttgta gcaaaaagaa ttctatacaa
351   aatcaaaaga atcattgtga atcgattcca agttgaagaa tagaatattc
401   attaatcaaa aataaaacca ttcactccag aatctgataa atctttcgaa
451   aaacggatta atcggacgag aataaagata gagtcccatt ctacatgtca
501   atatcaatac cgacaacaat gaaagttata gtaagaggaa aatccgtcga
551   cttgcaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details