JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972738.1
Database:	nt	Go to original record
Description:	Fumana procumbens isolate JH274 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Fumana procumbens
Original size:	1764 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	807	1368	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaat
101   gggcaatcct gagccaaatc ctgttttagg ggcagttata aaagcaataa
151   aaaaatagga taggtgcaga gactcgatgg aagttgttcg aacaaatggg
201   gttgaatggc tttgcgttgg taaaagaata cttatatcga aactccagca
251   aggataaacc tagcgaataa actatttcaa aaaaatgggg cacctattct
301   tttgatttgc ttataggtta tagcaaatca aaagaatcgt tgtgaatcga
351   ttccaaattg aagaatacat tattcattaa tcaaaaatca aaccattcac
401   tccacaatct gataaatctt ttgaaaaagg gattaatcgg acgagaataa
451   agatagagtc ccattctaca tgtcaatatc aataccgaca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details